e cadherin antibody sc 7870  (Santa Cruz Biotechnology)

Journal: Oncology Reports

Article Title: Development and characterization of a patient‑derived orthotopic xenograft of therapy‑resistant breast cancer

doi: 10.3892/or.2023.8536

Figure Lengend Snippet: Western blot analysis of breast cancer subtype-specific markers (A) PRa, PRb and epithelial and mesenchymal markers N-cadherin; (B) ERα, E-cadherin and Vimentin in tumor tissues from G1-G4 PDOXs. (C) Immunofluorescence analysis of the level of expression of ERα in PDOXs (G2 to G4) and DAPI for nuclear staining. PDOX, patient-derived orthotopic xenograft; ER, estrogen receptor; PR, progesterone receptor; G/Gen, generation.

Article Snippet: The cells were incubated overnight at 4°C with primary antibodies to the following proteins: estrogen receptor (ER)-α (cat. no. ab32063; Abcam), progesterone receptor (PR) (cat. no. ab8757; Cell Signaling Technology, Inc.), HER2 (cat. no. ab2428; Abcam), E-cadherin (cat. no. sc-7870; Santa Cruz Biotechnology, Inc.), N-cadherin (ab18203; Abcam), vimentin (sc-7558; Santa Cruz Biotechnology, Inc.), Ki-67 (sc-23900; Santa Cruz Biotechnology, Inc.), α-smooth muscle actin (SMA; ab5694; Abcam) and pan-cytokeratin (cat. no. C5992; Sigma-Aldrich; Merck Millipore) at 1:100 dilution.

Techniques: Western Blot, Immunofluorescence, Expressing, Staining, Derivative Assay

Journal: Oncology Reports

Article Title: Development and characterization of a patient‑derived orthotopic xenograft of therapy‑resistant breast cancer

doi: 10.3892/or.2023.8536

Figure Lengend Snippet: Immunofluorescence analysis of primary culture derived from PDOXs. (A and B) Established primary cultures were seeded on coverslips and fixed. (A) Primary cultures of G1 of PDOXs were stained with breast cancer subtype-specific markers, such as ERα, PRα and Ki-67 and epithelial and mesenchymal markers such as pan-CK, E-cadherin, vimentin and α-SMA. (B) Primary cultures of G4 of PDOXs were stained with breast cancer subtype-specific markers, ERα, PRα and Ki-67 and epithelial and mesenchymal markers such as Pan-CK N-Cadherin, Vimentin and α-SMA. PDOX, patient-derived orthotopic xenograft; SMA, smooth muscle actin; ER, estrogen receptor; PR, progesterone receptor; CK, cytokeratin.

Article Snippet: The cells were incubated overnight at 4°C with primary antibodies to the following proteins: estrogen receptor (ER)-α (cat. no. ab32063; Abcam), progesterone receptor (PR) (cat. no. ab8757; Cell Signaling Technology, Inc.), HER2 (cat. no. ab2428; Abcam), E-cadherin (cat. no. sc-7870; Santa Cruz Biotechnology, Inc.), N-cadherin (ab18203; Abcam), vimentin (sc-7558; Santa Cruz Biotechnology, Inc.), Ki-67 (sc-23900; Santa Cruz Biotechnology, Inc.), α-smooth muscle actin (SMA; ab5694; Abcam) and pan-cytokeratin (cat. no. C5992; Sigma-Aldrich; Merck Millipore) at 1:100 dilution.

Techniques: Immunofluorescence, Derivative Assay, Staining